Recombinant and native allergens
Significance of recombinant allergens
A selective allergy test prior to the specific immunotherapy requires the usage of highly pure allergens. In contrast to allergen extracts that contain a mixture of different allergens, recombinant allergens consist of only one protein. The usage of recombinant allergens helps to identify the protein to which specific immunoglobulines are present and cause the allergic reaction. During a specific immunotherapy (STI) a certain dosage of this pure allergen is administrated to the patient under medical supervision which barely cause symptoms (minimal dosage). This dosage is increased until habituation of immune system is achieved. STI takes 3 to 5 years and bodes a good success rate.
Recombinant allergens and native allergens
The difference between recombinant allergens and native allergens is the kind of manufacturing. To produce recombinant allergens genetically organisms are forced to assemble the allergen by themselves. The information about the allergen is transferred to the bacteria through vectors like plasmids. This plasmids carries the gene of this allergen and a reporter gene (for instance resistance to antibiotics), that helps to select those bacteria which sucessfully included plasmid. These bacteria are cultivated and recombinant allergens can be extracted from the supernatant.
Features of recombinant allergens
If stored at -18…-22°C the shelf life is 36 months.