Recombinant and native allergens

REF	Code	Description
51-672	d11	rDer p 1 Dermatophagoides pteronyssinus
51-673	d12	rDer p 2 Dermatophagoides pteronyssinus
51-676	d21	rDer p 10 Dermatophagoides pteronyssinus
51-677	d22	rDer p 23 Dermatophagoides pteronyssinus
51-674	d110	rDer f 1 Dermatophagoides farinae
51-675	d123	rDer f 2 Dermatophagoides farinae
52-108	e204	nBos d 6, BSA (Bovine serum albumin)
52-117	e220	nFel d 2, Cat serum albumin
52-118	e221	nCan f 3, Dog serum albumin
52-102	f67	nGal d 2, Ovalbumin
52-103	f68	nGal d 1, Ovomucoid
52-105	f69	nGal d 3, Conalbumin (Ovotransferrin)
52-119	f311	rDau c 1, Carrot
52-113	f352	rAra h 8, Peanut
52-114	f353	rGly m 4, Soya bean
52-115	f417	rApi g 1, Celery
52-112	f434	rMal d 1, Apple
52-110	g205	rPhl p 1, Timothy (Phleum pratense)
52-120	g206	rPhl p 2 Timothy (Phleum pratense)
52-111	g212	rPhl p 12, Timothy (Phleum pratense)
52-109	g215	rPhl p 5b, Timothy (Phleum pratense)
52-121	i12	nApi m4 Melittin
52-100	t215	rBet v1, Silver birch (Betula verrucosa)
52-106	t216	rBet v 2, Silver birch (Betula verrucosa)
52-107	t220	rBet v 4, Silver birch (Betula verrucosa)
52-116	w211	rPar j 2, Wall pellitory (Parietaria judaica)
52-101	w231	nArt v1, Mugwort (Artemisia vulgaris)
52-104	w233	nArt v3, Mugwort (Artemisia vulgaris)

Significance of recombinant allergens

A selective allergy test prior to the specific immunotherapy requires the usage of highly pure allergens. In contrast to allergen extracts that contain a mixture of different allergens, recombinant allergens consist of only one protein. The usage of recombinant allergens helps to identify the protein to which specific immunoglobulines are present and cause the allergic reaction. During a specific immunotherapy (STI) a certain dosage of this pure allergen is administrated to the patient under medical supervision which barely cause symptoms (minimal dosage). This dosage is increased until habituation of immune system is achieved. STI takes 3 to 5 years and bodes a good success rate.

Recombinant allergens and native allergens

The difference between recombinant allergens and native allergens is the kind of manufacturing. To produce recombinant allergens genetically organisms are forced to assemble the allergen by themselves. The information about the allergen is transferred to the bacteria through vectors like plasmids. This plasmids carries the gene of this allergen and a reporter gene (for instance resistance to antibiotics), that helps to select those bacteria which sucessfully included plasmid. These bacteria are cultivated and recombinant allergens can be extracted from the supernatant.

Features of recombinant allergens

If stored at -18…-22°C the shelf life is 36 months.
Recombinant allergens are intended for use with the Specific IgE kit of Astra Biotech GmbH.